Rovers picked up a point on the road away at Guiseley in the National League following a 0-0 draw at Nethermoor Park.
Micky Mellon switched Stephen McNulty back into the team in place of Ritchie Sutton in the only change from the starting XI from the lst game against Sutton United.
Rovers almost got off to a perfect start when Adam Buxton’s cross from the right found Andy Cook but the striker directed his header inches over the crossbar.
The hosts had a chance a few minutes later when a ball forward caught the Tranmere defence out with Reece Thompson charging down on goal but Scott Davies came off his line sharply to parry the ball away to safety.
Rovers were working into good positions without creating too many clear cut chances and then after the half hour mark, Liam Ridehalgh did well down the left and sent over a low cross which Cook got on the end of but steered his effort wide.
Balls went into the penalty area but the Guiseley rearguard remained solid and saw the game out goalless at the interval.
Cook had a volley saved by Joseph Green in the early stages of the second period while Thompson headed straight at Davies for the home side.
Ollie Norburn was showing an eye for goal and had couple of efforts from distance but Green again was equal to them.
For the final 16 minutes, James Alabi was introduced to replace Jay Harris.
The best chance of the second period came when Cook did well to get hold of the ball on halfway then threaded a through ball to Jack Dunn who raced in on goal but he dragged his shot across the face of goal and wide of the target.
Cook had an effort blocked inside the six yard box but it was not to be as Micky Mellon’s team had to settle for a point against resolute opposition.
Tranmere Rovers: Davies; Buxton, McNulty, McEveley, Ridehalgh; Hughes, Norburn; Jennings, Harris (Alabi 74), Dunn; Cook. Subs not used: Pilling, Mangan, Waring, Sutton.
Guiseley: Green; Williams, Atkinson, Hatfield, Lowe, Brown, Lawlor, Rooney, Purver, Thompson (Mulhern 90), Odejayi.